Absorption spectra and fluorescence of human hemoglobin in dimethyl sulfoxide and dimethylamine solvents

Document Type : Research Paper

Authors

porofosor

Abstract

In this work has investigated absorption and fluorescence spectra of the Human hemoglobin with using UV-Vis spectroscopy and fluorescence in several solvents (1.0 × 10-5M) where prepared in 5ml from Dimethyl sulfoxide and Dimethylamine solvents and After storing at 4°C for at least 24hr. The absorption spectra and Fluorescence results showed that electron transition and change in the initial nature of hemoglobin is due to solvent.----------------------------------------------------------------------------------------------------------------------------In this work has investigated absorption and fluorescence spectra of the Human hemoglobin with using UV-Vis spectroscopy and fluorescence in several solvents (1.0 × 10-5M) where prepared in 5ml from Dimethyl sulfoxide and Dimethylamine solvents and After storing at 4°C for at least 24hr. The absorption spectra and Fluorescence results showed that electron transition and change in the initial nature of hemoglobin is due to solvent.-----------------------------------------------------------------------------------------------------------------------------------In this work has investigated absorption and fluorescence spectra of the Human hemoglobin with using UV-Vis spectroscopy and fluorescence in several solvents (1.0 × 10-5M) where prepared in 5ml from Dimethyl sulfoxide and Dimethylamine solvents and After storing at 4°C for at least 24hr. The absorption spectra and Fluorescence results showed that electron transition and change in the initial nature of hemoglobin is due to solvent.

Keywords