نوع مقاله : مقاله پژوهشی
نویسندگان
دانشجو
چکیده
کلیدواژهها
عنوان مقاله [English]
Pakhvrhgndm disease caused by the fungus Gaeumannomyces graminis var. tritici is one of the most important wheat diseases Darrud. The biological control of this disease in different parts of the province 130 strains were isolated from wheat rhizosphere fluorescent. To the strains of Pseudomonas fluorescens with the ability to inhibit the growth of fungi, bacteria and fungi Ggt dual culture test on potato dextrose agar and King are performed. Of these, 21 strains capable of deterrence between 11 / 25-51 / 25% of isolates were superior in terms of prevention of fungal growth Ggt. Gene for antibiotic biosynthesis Fnazyn-1-Krbvksylykasyd (PCA), was performed by PCR using primers PCA2a and PCA3b. The results showed that 12 strains of antibiotic biosynthesis gene selected from 21 isolates had PCA. Production of qualitative methods to assess the antibiotic sediment dark green or crystal medium was used. Sown the antibiotic-producing strains Fnazyn-1-Krbvksylykasyd, more capable than others of disease control had Pakhvrhgndm the disease was 77-80%. By using these isolates significant increase in fresh weight of roots and aerial parts of the plants was observed. The results of this study suggests that antibiotic produced by strains of Pseudomonas fluorescens Fnazyn-1-Krbvksylykasyd, wheat take-all play role in reducing illness.
کلیدواژهها [English]
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